Simultaneous CE-MS monitoring

of amino acid and protein titer in the bioreactor

Now Available On Demand

Originally Recorded:
April 25, 2019

Speakers: Ying Zhou, Protein Analytical Scientist in Biologics CMC, Teva Branded Pharmaceutical Products R&D, Inc. and Aditya Kulkarni, Field Application Scientist, 908 devices

Sponsor: 908 devices

Moderator: Meagan Parrish, Senior Editor, Pharma Manufacturing

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Understanding bioreactor metabolomics and its relation to protein quality attribute are important aspects of the quality by design (QbD) approach for therapeutic proteins, therefore it is necessary to develop analytical methods for small molecule nutrient/metabolites and proteins from the culture media. Because of the different separation resin utilized, chromatographic separation usually requires different methods for measuring small molecules and proteins. As a result, the throughput could be hampered due to the time spent on developing and running two sets or more methods. In this webinar, we will present a novel approach for simultaneously monitoring small molecules and protein in bioreactor culture media using capillary electrophoresis-mass spectrometry (CE-MS). CE has enabled the simultaneous measurement because the background electrolyte is compatible with separating both small molecules and proteins. By using a commercial CE-MS system called ZipChip, we demonstrate the feasibility of monitoring amino acid and protein titer from clarified cell culture media.

 Key learning objectives: 

  • Learn how to simultaneously monitor small molecules and protein in a bioreactor using CE-MS.
  • Discover how ZipChip works and how it can be used for analysis of a wide variety of analytes from small molecules through intact proteins.
  • Understand how ZipChip takes CE-MS to the next level by achieving fast and efficient separations, while using very little sample and requiring minimal sample prep.
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Meet the Presenters


Headshot_Ying Zhou[1]Ying Zhou
Protein Analytical Scientist in Biologics CNC
Teva Branded Pharmaceutical Products R&D, Inc.

Ying Zhou is a protein analytical scientist in Biologics CMC at Teva Branded Pharmaceutical Products R&D, Inc. She earned her PhD in analytical chemistry from University of Michigan in 2015, with thesis work focusing on LC-MS quantitation of neuropeptides. Her responsibilities at Teva include the development of release, characterization  and process support methods for protein therapeutics. Ying is also involved in various CMC regulatory filing activities and analytical project leadership. Ying has authored 5 peer reviewed publications and has presented in multiple conferences in the past years.


Adi KulkarniAditya Kulkarni
Field Application Scientist
908 devices

Adi Kulkarni is a Field Applications Scientist at 908 devices. Prior to joining 908, Adi was an applications scientist at Bruker Daltonics where he worked on Bruker’s Q-TOF line of products. He received his PhD in Chemistry at University of Massachusetts Boston in 2011. After his PhD, Adi was a postdoctoral fellow at University of Southern California’s Loker Hydrocarbon Research Institute. He has authored 18 peer-reviewed articles.
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